We describe the external morphology and the internal oral features of the tadpole of Phyllomedusa itacolomi (Anura, Hylidae) from Serra de Ouro Branco, Municipality of Ouro Branco, State of Minas Gerais, Brazil. Tadpoles were found at mid water in a temporary pool, in an open montane meadow. Phyllomedusa itacolomi (Gosner's stage 37) is characterized by a tooth row formula 2(2)/3(1), labial tooth row P3 smaller than P1 and P2, marginal papillae of oral disc with wide dorsal and narrow ventral gaps. External morphological features were compared with other tadpoles of the Phyllomedusa hypochondrialis species group. Additionally, we provide information on egg clutches of P. itacolomi and P. megacephala.