The advertisement call is responsible for attracting females and helps the calling male to keep the acoustic territory by warning other males of its position. This call is species-specific and, because of this, it is frequently used in studies of taxonomy and phylogeny. In this study, we describe the advertisement call of Scinax similis recorded in Magé, State of Rio de Janeiro, Brazil, and compare it with the call presented by Haddad et al. in audio form. The advertisement call of Scinax similis is short and composed of one multipulsed note with two bands of frequency of similar intensity. The dominant frequency was found in the second band. The difference in the advertisement call of S. similis from the Municipality of Magé and the call presented by Haddad et al. can be interpreted as variation between populations and/or social context consequences. Between Brazilian species of the Scinax ruber clade, the call of S. similis is similar to that of S. auratus and S. juncae.