Habitats of three crayfish species, Procambarus (Pennides) gibbus (Muckalee Crayfish), Procambarus (Pennides) versutus (Sly Crayfish), and Procambarus (Pennides) spiculifer (White Tubercled Crayfish) were studied at nine localities in Marion County, GA. Major objectives of this year-long study were to compare habitats of these sister species and assess the current conservation status of the Muckalee Crayfish and the Sly Crayfish in Georgia. Sites were characterized by a one-time physical assessment of the substrate followed by bimonthly assessment of physicochemical conditions. Results indicated that Sly Crayfish habitats had lower pH and turbidity than habitats used by the other two species. Muckalee Crayfish habitat had higher gravel and coarse sand content and higher conductivity than those of the other two species. Captures indicated year-round activity of populations of each species across study locations; however, both the Muckalee Crayfish and the Sly Crayfish merit conservation status due to their limited ranges.