A juvenile Acipenser oxyrinchus desotoi (Gulf Sturgeon) was collected from the Santa Fe River, a major tributary of the Suwannee River, FL, on 6 December 2006. The Suwannee River is believed to contain the largest existing population of Gulf Sturgeon; however, our specimen is only the third Gulf Sturgeon collected from the Santa Fe River. Based on these observations, we believe that the Santa Fe River should be studied further to determine its importance as Gulf Sturgeon habitat, especially in the face of future management plans that may alter the hydrology of the system.