Although flehmen behavior is reported in felids, this display has been rarely documented in wild pumas. On 11 Nov. 2008, we recorded a female Puma concolor coryi (Florida Panther) exhibiting the flehmen response and scent marking in reaction to a baited trail camera site in Everglades National Park. The addition of scent lures to our camera sites increased the number of exposures per panther visit, enhancing the possibility of gender identification, an essential component of our annual survey.