The susceptibility of the spotted wing Drosophila suzukii Matsumura to four entomopathogenic fungal strains (Isaria fumosorosea Pf21, Pf17, Pf15, and Metarhizium anisopliae Ma59), was evaluated in vitro bioassays. The results showed that the percentage of mortality of the fly was 85% using the fungal strain Pf21 (85%), followed by Pf17, Pf15, and Ma59, with 60, 57, and 12% mortality, respectively. These results suggest that the application of these entomopathogenic fungal strains could be used as biological control agents of this insect that has recently become a pest of importance in Mexico.