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1 March 2015 Characterization of Cry Proteins in Native Strains of Bacillus thuringiensis and Activity Against Anastrepha ludens
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Abstract

Bacillus thuringiensis (Berliner) strains isolated from soil of citrus orchards were tested for insecticidal activity against the Mexican fruit fly, Anastrepha ludens (Loew), a key citrus pest in Mexico. From a total of 55 soil samples, 201 isolates were selected, for a total B. thuringiensis index of 0.66. The collection was characterized through light microscopy, polyacrylamide gel electrophoresis (SDS-PAGE), and PCR analysis detecting cry2, cry4, cry10, cry11, and cry19 genes. Of the 201 isolates, 51% produced ovoid crystals, 28% adhered to the spore, 15% were pleomorphic, 3% were bipyramidal, 2% cubic, and 1% was pyramidal type. Six colonies were positive for the cry10 gene and one for the cry19 gene. SDS-PAGE of spore-crystal preparations revealed seven electrophoresis patterns. These were bioassayed against Mexican fruit fly adults, obtaining maximum mortality of 28%.

S. Buentello-Wong, L. Galán-Wong, K. Arévalo-Niño, V. Almaguer-Cantú, and G. Rojas-Verde "Characterization of Cry Proteins in Native Strains of Bacillus thuringiensis and Activity Against Anastrepha ludens 1," Southwestern Entomologist 40(1), (1 March 2015). https://doi.org/10.3958/059.040.0102
Published: 1 March 2015
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