Seasonality and land-use change affect the composition of species. For this reason, the Culicidae family was studied in the central zone of Puebla where there are no records of study of this group of insects. Adult mosquitoes were sampled in an urban environment and in a conservation zone during three seasons: cold, dry, and rainy. In total, 11 species were identified in the genera Aedes (five), Culex (five), and Culiseta (one). Significant differences were found in Shannon diversity index when comparing the two environments during the rainy and cold seasons. Seasonality had an effect only in the conservation environment when comparing the rainy-cold and dry-cold seasons. In the Beta diversity index, the cold and dry communities in the urban environment had 100% similarity according to the Jaccard index. Culex salinarius Coquillett, 1904 and Culex stigmatosoma Dyar, 1907 had the widest range of humidity and temperature registered. Aedes guerrero (Berlin, 1969), Aedes quadrivittatus (Coquillett, 1902), Culex salinarius, Culex tarsalis Coquillett, 1896, and Culiseta particeps (Adams, 1903) were species not registered before in the state.