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1 January 2012 Selecting Nuclear Sequences for Fine Detail Molecular Phylogenetic Studies in Plants: A Computational Approach and Sequence Repository
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Abstract

When studying the phylogenetic relationships of closely related species, it is often difficult to find nuclear DNA regions that are both easy to amplify across taxa and contain informative characters. To address this problem, we have created a database of gene sequences specifically selected to greatly increase both the likelihood of amplification across the species of interest and the likelihood of retrieving nuclear regions that are variable among these species. This result is achieved by designing primers flanking putative intron splicing sites within highly conserved genes. Over 40 species were sampled spanning rosids (14 taxa), asterids (12 taxa), grasses (seven taxa) and other angiosperms, as well as several gymnosperms, a moss, and a green alga.

© Copyright 2012 by the American Society of Plant Taxonomists
Daniel C. Ilut and Jeff J. Doyle "Selecting Nuclear Sequences for Fine Detail Molecular Phylogenetic Studies in Plants: A Computational Approach and Sequence Repository," Systematic Botany 37(1), (1 January 2012). https://doi.org/10.1600/036364412X616576
Published: 1 January 2012
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