During 2008–2009, ticks were opportunistically collected from free-living lowland tapirs (Tapirus terrestris) and white-lipped peccaries (Tayassu pecari) during two studies of the ecology of Amazonian ungulates near or within two conservation areas in Madre de Dios, Peru. From tapirs, adults of the following tick species were collected: Amblyomma coelebs, Amblyomma incisum, Amblyomma latepunctatum, Amblyomma oblongoguttatum, Amblyomma ovale, and Amblyomma scalpturatum. Ticks collected from peccaries included adults of Amblyomma naponense and A. oblongoguttatum, nymphs of A. coelebs and A. latepunctatum, and larvae of Amblyomma sp. The 7 Amblyomma species collected in the present study have been previously reported in Peru. Although it is a common parasite of tapirs and/or peccaries in the Neotropical Region, we provide the first report of A. oblongoguttatum on tapirs in Peru. In addition, we provide the first report of A. coelebs and A. latepunctatum nymphs on peccaries. For nymphs of the latter tick species, this is the first host record.