Bio-ecological parameters of the two-spotted spider mite (TSSM), Tetranychus urticae Koch and its predators depend on the quality of its host plants. In this study, the life table parameters of TSSM and its main predator Phytoseiulus persimilis Athias-Henriot during different generations (1st, 5th, and 10th generations) were determined on the susceptible (‘Beth-Alpha’) and resistant (‘HED’) greenhouse cucumber cultivars. The results indicated that the longest total development time of TSSM was allocated to the first generation on the susceptible cultivar, but there was no significant difference among different generations on the resistant cultivar. The total development time of the 1st and 10th generations of P. persimilis on each of the susceptible and resistant cultivars had not significant difference. Total fecundity of TSSM was higher in the 10th generation on both cultivars tested than that of the former generations. However, the total fecundity of the predator drastically reduced after long-term rearing (10 generations) on both cultivars. The intrinsic rate of increase (r) of the 1st, 5th, and 10th generations of TSSM on the susceptible cultivar was 0.178, 0.238 and 0.319 day-1, respectively, and 0.157, 0.166 and 0.168 day-1 on the resistant cultivar, respectively which indicates the significantly negative effect of the resistant cultivar on this parameter. In P. persimilis, the value of the intrinsic rate of increase in the first and tenth generations on the susceptible cultivar was 0.275 and 0.267 day-1, respectively, and on the resistant cultivar was 0.244 and 0.210 day-1, respectively. By understanding these interactions in the long term, we will be able to develop suitable strategies for biological control of TSSM on cucumber.