Translator Disclaimer
1 July 1999 Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and Mice
Author Affiliations +
Abstract

In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense, mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization. Quick DNA extraction method could take the place of phenol-chloroform method to prepare DNA template when detecting the rickettsiae in mice and patients' blood by PCR. De-inhibitor quick DNA extraction method could improve the detection rate of rickettsiae from chigger mites.

© 1999 Systematic & Applied Acarology Society
Zhiyong Xi, Jiacan Li, Xiaoying Zheng, and Xie Yan "Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and Mice," Systematic and Applied Acarology 4(1), 15-20, (1 July 1999). https://doi.org/10.11158/saa.4.1.3
Accepted: 1 March 1999; Published: 1 July 1999
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top