The enzyme-linked immunosorbent assay (ELISA) is a powerful immunological technique for quantifying small amounts of compounds and has been used in research and clinical settings for years. Although there are laboratory exercises developed to introduce the ELISA technique to students, their ability to promote student learning has not been thoroughly assessed. We found that a commercially available ELISA kit increased student performance on pre- and post-tests in three undergraduate college courses, especially in those taught to general-education students. Student confidence levels about ELISA methodology, as well as comfort level in performing the technique, increased significantly in both generaleducation and biology-major courses.
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Vol. 74 • No. 8