We outline protocols for producing slant-minis (SLINIs) and mini-deeps (MEEPs) and examples of their use in simple microbiology experiments suitable for high school students. The principal benefits of these protocols are decreased cost associated with significantly reduced media use; easier, less expensive disposal of waste; and increased safety by switching from glass tubes to nonbreakable plastic. Additionally, these protocols can be utilized as hands-on reinforcement of concepts of pH, metabolism, and digestion.
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Vol. 75 • No. 8