Translator Disclaimer
1 March 2015 Grocery Store Genetics: A PCR-Based Genetics Lab that Links Genotype to Phenotype
Author Affiliations +
Abstract

Instructors often present Mendelian genetics and molecular biology separately. As a result, students often fail to connect the two topics in a tangible manner. We have adopted a simple experiment to help link these two important topics in a basic biology course, using red and white onions bought from a local grocery store. A lack of red coloration in white onions is a result of one or more mutations in the color production pathway. This mutation can be seen by the use of polymerase chain reaction (PCR) followed by gel electrophoresis. An absence of an amplified PCR product for one of the genes necessary for color production is associated with a lack of color production — an obvious trait in white onion. The students are able to “see” the difference at the DNA level between the red and white onion.

©2015 by National Association of Biology Teachers. All rights reserved. Request permission to photocopy or reproduce article content at the University of California Press's Rights and Permissions Web site at www.ucpressjournals.com/reprintinfo.asp.
Betsy J. Briju and Sarah E. Wyatt "Grocery Store Genetics: A PCR-Based Genetics Lab that Links Genotype to Phenotype," The American Biology Teacher 77(3), 211-214, (1 March 2015). https://doi.org/10.1525/abt.2015.77.3.10
Published: 1 March 2015
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top