Creating a Reliable, Cost-Effective ELISA Simulation

Julie Olson; Michael Amolins; Peter Vitiello

doi:10.1525/abt.2017.79.4.301

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1 April 2017 Creating a Reliable, Cost-Effective ELISA Simulation

Julie Olson, Michael Amolins, Peter Vitiello

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The American Biology Teacher, 79(4):301-304 (2017). https://doi.org/10.1525/abt.2017.79.4.301

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a fundamental laboratory technique with direct applications across scientific research and clinical diagnostics as well as everyday life. Unfortunately, many challenges exist that inhibit both its introduction and implementation in the high school biology classroom. We present a reliable yet inexpensive way of effectively simulating this assay, allowing student exposure to several advanced topics, including immunodetection, clinical diagnostics, and qualitative and quantitative colorimetric analysis.

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Julie Olson, Michael Amolins, and Peter Vitiello "Creating a Reliable, Cost-Effective ELISA Simulation," The American Biology Teacher 79(4), 301-304, (1 April 2017). https://doi.org/10.1525/abt.2017.79.4.301

Published: 1 April 2017

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