Phylogenetics plays a central role in understanding the evolution of life on Earth, and as a consequence, several active teaching strategies have been employed to aid students in grasping basic phylogenetic principles. Although many of these strategies have been designed to actively engage undergraduate biology students at the freshman level, less attention is given to designing challenges jor advanced students. Here, I present a project-based learning (PBL) activity that was developed to teach phylogenetics for junior and senior-level biology students. This approach reinforces the theories and concepts that students have learned in their freshman courses along with incorporating Bioinformatics, which is essential for teaching zoology in the 21st century.