There is a paucity of information regarding the feeding habits of the Southern flying squirrel Glaucomys volans. We investigated dietary preference of G. volans by computing the mean daily rate of ingested energy for five food resources: fruits of flowering dogwood Cornus florida; acorns of white oak Quercus alba, water oak Q. nigra, and black oak Q. velutina; and nuts of pignut hickory Carya glabra. The preferred food of G. volans was Q. alba, constituting 49.7% of their mean daily diet. Q. velutina and Q. nigra were ranked next in dietary preference. The least preferred food types were the fruits of C. florida, and nuts of C. glabra. There was no significant difference when comparing sex with food preference and sex with caloric intake.