CO₂ flux from soil is a significant component of total atmospheric carbon, as well as an indicator of soil biological health, a useful tool for evaluating the impact of agricultural practices, and a key variable relevant to global climate change. A year-long study was conducted to evaluate subterranean termite contribution to soil CO₂ flux on Oklahoma's Joseph H. Williams Tallgrass Prairie Preserve. Gas samples were extracted from metal flux chambers located within soil plots containing naturally occurring populations of foraging subterranean termites and from two different types of control plots without termites. Results indicate that CO₂ flux during January, March, April, June, July, and December was similar among all three treatments. However, CO₂ flux in control plots during May was significantly greater compared with termite-active plots. Overall CO₂ flux from both termite-infested and termite-free plots was great enough to obscure CO₂ contributions from subterranean termites alone. Therefore, although total normal baseline CO₂ emissions from soil were measured, the specific amount of CO₂ that termites alone contributed to this flux could not be accurately determined.