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1 January 2010 Molecular Cloning, Characterization, and Expression Analysis of a Novel sema-2a Homologue in Polyrhachis vicina (Hymenoptera: Formicidae)
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Abstract

The semaphorin gene family plays important roles in axonal guidance in vertebrates and invertebrates. Semaphorin 2a, a member of the semaphorin family, belongs to class 2, which is found only in invertebrates. In our study, semaphorin 2a was cloned from the ant Polyrhachis vicina Roger. The full length of P. vicina semaphorin 2a (Pv-sema-2a) is 2763 base pairs (bp) and it contains a 5′-untranslated region (UTR) 92 bp long and a 3′-UTR 521 bp long. The open reading frame of Pv-sema-2a encodes a 716-amino-acid protein with a predicted molecular mass of 81.1 kilodaltons. Real-time quantitative reverse-transcription — polymerase chain reaction indicated that Pv-sema-2a mRNA. is differentially expressed during P. vicina development, in the whole bodies as well as the heads of different castes. The high mRNA levels in embryos and pupae suggest that Pv-sema-2a plays an important role in ant development.

© 2010 Entomological Society of Canada
Jing Luo, Geng-Si Xi, Shu-Min Lü, Ke Li, and Qing Li "Molecular Cloning, Characterization, and Expression Analysis of a Novel sema-2a Homologue in Polyrhachis vicina (Hymenoptera: Formicidae)," The Canadian Entomologist 142(1), 1-13, (1 January 2010). https://doi.org/10.4039/n09-046
Received: 2 May 2009; Accepted: 1 September 2009; Published: 1 January 2010
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