Labena Cresson is a large Gondwanan genus best represented in the Neotropical area. We describe three new species in the Neotropical Labena striata species group: Labena gauldiana sp. nov. from Costa Rica and Mexico, Labena yucatanica sp. nov. from Mexico, and Labena iquitosica sp. nov. from Peruvian Amazonia. These species are among the largest and most spectacular Neotropical Ichneumonidae ever collected. We also provide additional descriptive remarks regarding Labena striata Townes and Townes (including intraspecific variation) and an identification key to the six species of the striata group.