Here we describe the association of Enema pan (F., 1775) (Scarabaeidae: Dynastinae: Oryctini) with bamboo plants in the genus Guadua Kunth (Poaceae: Bambuseae) in a lowland rainforest in southeastern Peru. Mounds at the base of Guadua stems and underground tunnels over one meter long are constructed by adults. Males were found inside these tunnels and in mounds at the base of bamboo stems where they shred and feed on bamboo sap. Males were observed perching at the top of opened mounds only at night, waving their antennae. This study provides the first description of the burrows of E. pan and its association with bamboo plants in southwestern Amazonia.