Sternidius alpha (Say) was previously recognized as having several subspecies that were subsequently synonymized. We examined two variants of S. alpha that seem to be morphologically similar except for their color. Specimens of S. alpha collected in Indiana show two different common color patterns depending on the locality. DNA was extracted from 14 individuals. Phylogenetic analysis using a 648 bp partial sequence from the barcoding region of the cytochrome oxidase I gene revealed three different clades.
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