Tissues of lycosid spiders were studied for RNA distributions with the basic dye Azure B. Changes in the basic proteins associated with DNA during spermiogenesis were identified with alkaline fast green staining after DNA extraction with trichloroacetic acid and by cytochemical tests for arginine. Tissue glycoproteins of the gut diverticula and the ducts of silk glands were resistant to diastase digestion and required periodic acid hydrolysis to localize reaction products with the Schiff reagent for aldehydes. Spiders possess novel types of cells that are in need of further study and may be useful as models for developmental biology.
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