Two new species of the spider genus Ochyrocera Simon 1891 are described from Mexico. Ochyrocera juquila new species was collected under moist rotten logs and hollow trunks on a thick bed of pine needles in oak-pine forests located in a mountain range south of the city of Oaxaca at 1400–2700 m elev. Ochyrocera juquila resembles O. quinquevittata Simon 1891 from the Island of St. Vincent, in the angular shape of the embolus, which in the new species is V-shaped and in O. quinquevittata is L-shaped. Ochyrocera chiapas new species, was collected under rotten trunks and hollow trunks in abundant leaf litter in the tropical, humid Lacandona rainforest region located in eastern Chiapas, near the border with Guatemala. The species occurs at 160–260 m elev. Ochyrocera chiapas resembles O. arietina Simon, 1891 from the island of St. Vincent, in the similar shape of the embolus and distal apophysis of the cymbium, but in O. chiapas the embolus is more strongly curved and directed toward the distal part of the tibiae forming a “D”; in O. arietina the embolus is not as strongly curved as in O. chiapas. In both species, males and females were collected near each other; the females carried their egg sacs with their chelicerae. A key to the four known Mexican species is provided.