The chromosome diploid number (2n) and the sex chromosome system in males of five species belonging to the families Gnaphosidae and Lycosidae were determined as 2n = 22 (20 X1X2) and 2n = 28 (26 X1X2), respectively. Nomisia conigera (Spassky 1941), Haplodrassus morosus (O. Pickard-Cambridge 1872) and Haplodrassus dalmatensis (L. Koch 1866) have 10 autosomal bivalents and two univalent sex chromosomes, while Pardosa bifasciata (C.L. Koch 1834) and Arctosa cinerea (Fabricius 1777) have 13 autosomal bivalents and two univalent sex chromosomes during first meiotic stages of prophase I and metaphase I. All species have acrocentric chromosomes and chiasmatic meiosis.
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