We evaluated spider guild abundance and vegetation complexity in riparian forests in southern Brazil in four distinct river basins over 2 yr. We compared spider guild abundance among rivers, habitats (edges vs. forest interior), and against vegetation complexity. We also compared spider assemblages between juvenile and adults in terms of guilds. Of 42,057 spiders sampled, 79% were juveniles and 21% were adults. Space-web weaving was the most abundant guild whereas cursorial hunters was the least abundant one. Weaving spiders dominated over hunters (59% vs 40.58%, respectively). Thirty-four families were recorded; ambush hunters totaled 11 families; space web sheet builders and hunting runners had eight families each and there were seven families for orbicular web builders. Space web sheet builders dominated on all levels: among rivers and habitats (edges and forest interior). Unexpectedly, spider guilds did not seem to be influenced by environmental complexity, given the variables measured, under a Canonical Correspondence Analysis. There was an interaction between guild relative abundance and ontogeny, since the proportion of the space web sheet builders guild among adult spiders was larger than the proportion among juveniles, with a decrease in proportion of adults especially for hunting runners.