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1 November 2014 Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing
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Abstract

We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura - Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.

The American Arachnological Society
Enric Planas, Laia Bernaus, and Carles Ribera "Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing," The Journal of Arachnology 42(3), 315-317, (1 November 2014). https://doi.org/10.1636/B13-88.1
Received: 19 December 2013; Published: 1 November 2014
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