An Indexed Genomic Library for Paramecium Complementation Cloning

KELLER ANNE-MARIE; COHEN JEAN

doi:10.1111/j.1550-7408.2000.tb00001.x

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KELLER ANNE-MARIE,¹ COHEN JEAN¹

¹Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Avenue de la Terrasse

Abstract

Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.

Citation Download Citation

KELLER ANNE-MARIE and COHEN JEAN "An Indexed Genomic Library for Paramecium Complementation Cloning," The Journal of Eukaryotic Microbiology 47(1), 1-6, (1 January 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00001.x

Received: 15 July 1999; Accepted: 22 September 1999; Published: 1 January 2000