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1 March 2000 Use of Micromanipulation and “Feeder Layers” to Clone the Oyster Pathogen Perkinsus marinus
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Abstract

Genetic and biochemical characterization of microbes often requires the use of clonal cultures. A method to clone the oyster parasite Perkinsus marinus is described. Individual cells are isolated via micromanipulation and maintained above an actively proliferating “feeder layer” of P. marinus on a 0.45-μm membrane. Extracellular products released from the proliferating feeder layer can diffuse across the membrane and bathe the isolated cell, stimulating it to proliferate. The method is relatively simple and should be applicable to most protists that can be cultured in the laboratory.

DAVID BUSHEK, RUSSELL A. HOLLEY, and KIMBERLY S. REECE "Use of Micromanipulation and “Feeder Layers” to Clone the Oyster Pathogen Perkinsus marinus," The Journal of Eukaryotic Microbiology 47(2), (1 March 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00027.x
Received: 22 April 1999; Accepted: 2 November 1999; Published: 1 March 2000
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