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1 January 2001 Phosphatidylinositol-Specific Phospholipase C (PI-PLC) Cleavage of GPI-Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes
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Abstract

Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we were able to show that trypomastigotes treated with PI-PLC initiate the process of flagellum remodeling by 30 sec after contact with the enzyme and amastigote-like forms are detected as early as 10 min after PI-PLC treatment. Scanning and transmission electron microscopy indicate that trypomastigotes undergo a previously undescribed process of flagellum circularization and internalization. Analysis of the flagellar complex with monoclonal antibody 4D9 shows heterogeneous labeling among the parasites, suggesting a remodeling of these molecules. After PI-PLC treatment, parasites rapidly lose the surface marker Ssp-3 and 24 h post-treatment they begin to exhibit a circular nucleus and a rod-shaped kinetoplast. By flow cytometry analysis and confocal microscopy, the Ssp-4 amastigote-specific epitope can be detected on the parasite surface. This indicates that the release of trypomastigote GPI-anchored molecules by exogenous PI-PLC in vitro can trigger morphological changes.

RENATO A. MORTARA, LINDAMAR M. S. MINELLI, FILIP VANDEKERCKHOVE, VICTOR NUSSENZWEIG, and F. JUAREZ RAMALHO-PINTO "Phosphatidylinositol-Specific Phospholipase C (PI-PLC) Cleavage of GPI-Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes," The Journal of Eukaryotic Microbiology 48(1), (1 January 2001). https://doi.org/10.1111/j.1550-7408.2001.tb00413.x
Received: 20 June 2000; Accepted: 24 September 2000; Published: 1 January 2001
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