Simple, Filter-based PCR Detection of Thelohania solenopsae (Microspora) in Fire Ants (Solenopsis invicta)

KAREN F. SNOWDEN; KATHLEEN S. LOGAN; S. BRADLEIGH VINSON

doi:10.1111/j.1550-7408.2002.tb00226.x

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1 November 2002 Simple, Filter-based PCR Detection of Thelohania solenopsae (Microspora) in Fire Ants (Solenopsis invicta)

KAREN F. SNOWDEN, KATHLEEN S. LOGAN, S. BRADLEIGH VINSON

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The J. of Eukaryotic Microbiology, 49(6):447-448 (2002). https://doi.org/10.1111/j.1550-7408.2002.tb00226.x

Abstract

Thelohania solenopsae is a microsporidian parasite that may serve as a biological control agent for the red imported fire ant, Solenopsis invicta. A rapid, filter-based PCR amplification method detecting a portion of the small-subunit ribosomal RNA gene was developed to facilitate field studies detecting the parasite in fire ants. Processing ant homogenates with a commercially available membrane-based system, FTA Classic Card technology, compared favorably with traditional DNA extraction and PCR amplification methods. As few as 100 spores were detected. The FTA membrane system is a simple, extraction-free method for detecting T. solenopsae in fire ants, and allows for easy archival storage of DNA samples.

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KAREN F. SNOWDEN, KATHLEEN S. LOGAN, and S. BRADLEIGH VINSON "Simple, Filter-based PCR Detection of Thelohania solenopsae (Microspora) in Fire Ants (Solenopsis invicta)," The Journal of Eukaryotic Microbiology 49(6), 447-448, (1 November 2002). https://doi.org/10.1111/j.1550-7408.2002.tb00226.x

Received: 8 February 2002; Accepted: 17 August 2002; Published: 1 November 2002

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