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1 July 2007 In Vitro Asymbiotic Germination, Protocorm Development, and Plantlet Acclimatization of Aplectrum Hyemale (Muhl. ex Willd.) Torr. (Orchidaceae)
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Abstract

A method to significantly improve the germination and development of plantlets of Aplectrum hyemale was developed. Seeds of this terrestrial orchid were collected in a natural stand, surface disinfected and grown in vitro on a gellified growth medium in absence of symbiotic fungi. Seeds were sowed whole or after excision of the seed coat. Embryos that were removed from the seed coat showed a significant increase in germination and survival percentages compared to whole seeds. Embryos grew to form a ramified protocorm, irrespective of seed treatment, followed by plantlet formation. Plantlets were successfully acclimatized and transferred to soil conditions. Asymbiotic in vitro culture is therefore shown as a potential tool to produce viable plantlets for use in natural site restoration.

Denis Lauzer, Sébastien Renaut, Marc St-Arnaud, and Denis Barabé "In Vitro Asymbiotic Germination, Protocorm Development, and Plantlet Acclimatization of Aplectrum Hyemale (Muhl. ex Willd.) Torr. (Orchidaceae)," The Journal of the Torrey Botanical Society 134(3), 344-348, (1 July 2007). https://doi.org/10.3159/1095-5674(2007)134[344:IVAGPD]2.0.CO;2
Received: 29 January 2007; Published: 1 July 2007
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