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1 July 2007 In Vitro Asymbiotic Germination, Protocorm Development, and Plantlet Acclimatization of Aplectrum Hyemale (Muhl. ex Willd.) Torr. (Orchidaceae)
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Abstract

A method to significantly improve the germination and development of plantlets of Aplectrum hyemale was developed. Seeds of this terrestrial orchid were collected in a natural stand, surface disinfected and grown in vitro on a gellified growth medium in absence of symbiotic fungi. Seeds were sowed whole or after excision of the seed coat. Embryos that were removed from the seed coat showed a significant increase in germination and survival percentages compared to whole seeds. Embryos grew to form a ramified protocorm, irrespective of seed treatment, followed by plantlet formation. Plantlets were successfully acclimatized and transferred to soil conditions. Asymbiotic in vitro culture is therefore shown as a potential tool to produce viable plantlets for use in natural site restoration.

Denis Lauzer, Sébastien Renaut, Marc St-Arnaud, and Denis Barabé "In Vitro Asymbiotic Germination, Protocorm Development, and Plantlet Acclimatization of Aplectrum Hyemale (Muhl. ex Willd.) Torr. (Orchidaceae)," The Journal of the Torrey Botanical Society 134(3), (1 July 2007). https://doi.org/10.3159/1095-5674(2007)134[344:IVAGPD]2.0.CO;2
Received: 29 January 2007; Published: 1 July 2007
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