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1 June 2017 Isolation and Characterization of Polymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
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Abstract

Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds.

Juliana Menger, Michael Gerth, Jasmin Unrein, Klaus Henle, and Martin Schlegel "Isolation and Characterization of Polymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)," The Wilson Journal of Ornithology 129(2), 407-411, (1 June 2017). https://doi.org/10.1676/16-062.1
Received: 27 April 2016; Accepted: 1 October 2016; Published: 1 June 2017
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