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1 September 2017 Isolation and Characterization of 18 Polymorphic Microsatellite Loci for the Endangered New Zealand Black-Fronted Tern (Chlidonias albostriatus)
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Abstract

Eighteen polymorphic microsatellite loci were isolated and characterized from the endangered Black-fronted Tern (Chlidonias albostriatus), a species endemic to New Zealand. The loci were initially tested on seven individuals spanning the entire breeding range and then for a larger dataset of 345 samples covering 30 colonies from throughout the species' range. The number of alleles per loci ranged from 4 to 24, and observed and expected heterozygosity ranged between 0.46 and 0.95 and 0.45 to 0.86, respectively. One locus showed significant heterozygote deficit and appears to be sex-linked. The similar annealing temperatures across loci and the wide fragment sizes allowed multiplex polymerase chain reaction and rapid multilocus genotyping. The microsatellite markers will be useful tools for further investigation into the genetic diversity, population structure and effective population size of this endangered species and for informing conservation management.

Ann-Kathrin V. Schlesselmann and Bruce C. Robertson "Isolation and Characterization of 18 Polymorphic Microsatellite Loci for the Endangered New Zealand Black-Fronted Tern (Chlidonias albostriatus)," Waterbirds 40(3), (1 September 2017). https://doi.org/10.1675/063.040.0311
Received: 6 February 2017; Accepted: 1 March 2017; Published: 1 September 2017
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