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1 March 2015 EPSPS Gene Amplification is Present in the Majority of Glyphosate-Resistant Illinois Waterhemp ( Amaranthus tuberculatus) Populations
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With the frequency of glyphosate-resistant waterhemp increasing throughout the Midwest, the identification of resistant populations has become important for managing this species. However, high-throughput screening for glyphosate resistance in the greenhouse is tedious and inefficient. Research was conducted to document the occurrence of glyphosate-resistant waterhemp throughout the state of Illinois, and to determine whether a molecular assay for 5-enolypyruvyl-shikimate-3-phosphate synthase (EPSPS) gene amplification can be used as an alternative means to detect resistant populations. Populations throughout the state of Illinois were collected in 2010 and screened for glyphosate resistance using a whole-plant assay in a greenhouse, and survivors were examined for EPSPS gene amplification. Of 80 populations investigated, 22 were glyphosate resistant based on the greenhouse screen, and gene amplification was identified in 20 (91%) of the resistant populations. Although there are multiple mechanisms for glyphosate resistance in waterhemp, a molecular test for EPSPS gene amplification provides a rapid alternative for identification of glyphosate resistance in most populations.

Nomenclature: Glyphosate; common waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer).

Con el incremento en la frecuencia de Amaranthus tuberculatus resistente a glyphosate a lo largo del Medio oeste, la identificación de poblaciones resistentes se ha hecho importante para el manejo de esta especie. Sin embargo, la evaluación rápida y de cantidades grandes de muestras para detectar resistencia a glyphosate en el invernadero es tediosa e ineficiente. Se realizó una investigación para documentar la frecuencia de A. tuberculatus resistente a glyphosate a lo largo del estado de Illinois, y determinar si una prueba molecular evaluando la amplificación del gen de 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) puede ser usado como una alternativa para detectar poblaciones resistentes. Las poblaciones a lo largo del estado de Illinois fueron colectadas en 2010 y evaluadas por resistencia a glyphosate usando una prueba con plantas enteras en un invernadero, y las plantas sobrevivientes fueron examinadas para detectar la amplificación del gen EPSPS. De las 80 poblaciones investigadas, 22 fueron resistentes a glyphosate con base en la evaluación en el invernadero, y la amplificación del gen se identificó en 20 (91%) de las poblaciones resistentes. Aunque existen múltiples mecanismos de resistencia a glyphosate en A. tuberculatus, una prueba molecular de amplificación del gen EPSPS brinda una alternativa rápida para la identificación de resistencia a glyphosate en la mayoría de las poblaciones.

Laura A. Chatham, Chenxi Wu, Chance W. Riggins, Aaron G. Hager, BRYAN G. YOUNG, Gordon K. Roskamp, and PATRICK J. TRANEL "EPSPS Gene Amplification is Present in the Majority of Glyphosate-Resistant Illinois Waterhemp ( Amaranthus tuberculatus) Populations," Weed Technology 29(1), 48-55, (1 March 2015).
Received: 4 January 2014; Accepted: 1 September 2014; Published: 1 March 2015

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