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1 December 2003 A RAPID METHOD FOR IDENTIFYING THE ORIGIN OF NORTH AMERICAN PHRAGMITES POPULATIONS USING RFLP ANALYSIS
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Abstract

The common reed, Phragmites australis, is a common feature in wetlands across North America. Recent studies have suggested that the widespread invasions of this species may be due to the introduction of a non-native strain from EurAsia. Since native populations persist in many areas where introduced populations are also found, a method for distinguishing population types is needed to facilitate management of the species. A restriction fragment length polymorphism (RFLP) assay was developed to distinguish native, non-native, and Gulf Coast type populations of Phragmites from each other. Two amplified non-coding chloroplast DNA regions are each cut with one restriction enzyme, allowing the distinction of native, non-native and Gulf Coast haplotypes from each other. When used together, these cut sites provide a low cost, rapid way of determining the origin of Phragmites populations.

Kristin Saltonstall "A RAPID METHOD FOR IDENTIFYING THE ORIGIN OF NORTH AMERICAN PHRAGMITES POPULATIONS USING RFLP ANALYSIS," Wetlands 23(4), 1043-1047, (1 December 2003). https://doi.org/10.1672/0277-5212(2003)023[1043:ARMFIT]2.0.CO;2
Received: 24 June 2002; Accepted: 1 June 2003; Published: 1 December 2003
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