Allium symiacum (Amaryllidaceae), a new species from Symi Island (SE Aegean, Greece)

Abstract: Allium symiacum Galanos & Tzanoud., from the island of Symi (SE Aegean, Greece), is described as a species new to science. It is an autumn-flowering, single-island endemic species of A. sect. Codonoprasum (Amaryllidaceae) and is classified as Critically Endangered according to IUCN Red List categories and criteria. Considering the morphological and karyological characters of the new species, its possible relationships to other autumnal species of A. sect. Codonoprasum distributed in the E Mediterranean area are discussed. Citation: Galanos Ch. J. & Tzanoudakis D. 2017: Allium symiacum (Amaryllidaceae), a new species from Symi Island (SE Aegean, Greece). — Willdenowia 47: 107–113. doi: https://doi.org/10.3372/wi.47.47202 Version of record first published online on 17 May 2017 ahead of inclusion in August 2017 issue.


Introduction
Allium L. (Amaryllidaceae) is considered as one of the most species-rich genera of flowering plants, as it comprises more than 960 species (Govaerts & al. 2005(Govaerts & al. -2014. In Flora europaea (Stearn 1980; see also Stearn 1978), 47 Allium species were recorded as occurring in Greece, and the Greek area was regarded as an evolutionary centre for the genus (Stearn 1981;Tzanoudakis & Vosa 1988). This view is further supported by newer floristic data, according to which, the genus Allium is represented in Greece by more than 100 species (Dimopoulos & al. 2013;Brullo & al. 2015;Tzanoudakis & Trigas 2015;Trigas & al. 2017). The doubling of species number within the last four decades, mainly because of the description of numerous new species, indicates the amplitude of diversity of the genus in Greece.
It is worth noting that among these new Allium taxa, two, often overlapping, groups are well represented. The first one consists of species of A. sect. Codonoprasum Rchb., the members of which show a remarkable diversity regarding their morphology, ploidy level, habitat traits and flowering period. The second group consists of species particularly characterized by an autumn-flowering period and a life cycle in which the dormancy phase is almost absent (Tzanoudakis & Kypriotakis 1993).
The Allium species described here from the island of Symi is a member of A. sect. Codonoprasum, is autumn-flowering and was collected in the framework of the floristic explorations and studies of the authors in the Aegean islands.
The island of Symi was visited by the first author in November 2015. During the fieldwork, a small Allium population, with some individuals still in flower, was found growing on a calcareous coastal slope. The autumnal flowering period and the obvious morphological differences of the individuals from all known autumn-flowering Allium species of Greece indicated an interesting 108 Galanos & Tzanoudakis: Allium symiacum from Symi Island, Greece new finding. Living bulbs and herbarium specimens were collected for cultivation and further studies. Careful and thorough examination of the collected material lead us to the conclusion that it is distinct from all other members of A. sect. Codonoprasum and it is described here as a species new to science.
Symi is one of the small but inhabited islands of the Dodekanisa island complex (Dodecanese, SE Aegean region) and it is located 22 km N of Rodos (Rhodes) and 6.75 km W of the nearest Turkish coast (36°36'N, 27°50'E, Fig. 1). It covers an area of 58 km², has a coastline of 84 km and includes five main settlements with a total population of c. 2500 permanent inhabitants. It is mostly mountainous and rocky, and its highest elevation (Vigla peak) is 616 m. According to the cli-matic diagrams of the Hellenic National Meteorological Service (http://www.hnms.gr), the climate of the island is similar to that of Rodos, i.e. semi-arid Mediterranean, with short, mild and wet winters, followed by long, hot and dry summers.
The vegetation of the island mainly consists of phrygana communities and remnants of pre-existing conifer (Cupressus L. and Pinus L.) woodlands, as well as sclerophyllous and deciduous forests. The latter, however, are nowadays limited by human impact to the foot of the hills and the valleys (Kagiampaki 2011). According to the literature (Carlström 1987;Chilton 1999;Galanos 2016;Strid 2016), the vascular flora of Symi known until the present study amounts to more than 600 species.

Material and methods
The floristic exploration in the island of Symi, as well as the collection of herbarium specimens and living bulbs, took place in November 2015 and October 2016, after obtaining official permission (see Acknowledgements). The bulbs were cultivated at the experimental botanical garden of the University of Patras for karyological and morphological studies. For the karyological analyses, root tips from potted bulbs were pre-treated in a-bromonaphthalene for c. 8 hours at 4°C. Fixation, staining and chromosome measurements and construction of the karyogram follow Tzanoudakis (1983). Diagnosis -Allium symiacum is an autumnflowering species of A. sect. Codonoprasum. It differs from other autumn-flowering species of the A. paniculatum subgroup known from Greece mainly by its smaller perianth with exserted stamens and from the species of the A. flavum L. and A. stamineum Boiss. subgroups (which are also characterized by exserted stamens) by its greenish white flowers appearing in autumn.
Phenology -Flowering from late September to the middle November; the first mature seeds appear almost one month later, from October to late November.  Etymology -The specific epithet is derived from the Greek adjective symiakós (συμιακός), which means originating from the island of Symi, where the species was discovered.
Karyology -In all the material examined the diploid chromosome number 2n = 2x = 16 was found. Regarding the chromosome size and morphology, the data available suggest that the new species is characterized by a symmetrical karyotype, since anisobrachial (sm or st) chromosomes have not been observed in the metaphase plates, while the eight metacentric chromosomes of the haploid complement do not show significant size differences (Fig. 4). It is also worth noting that chromosomes with nucleolar organizers (SAT-chromosomes) have not been observed in the material examined. While the basic chromosome number x = 8 characterizes Allium sect. Co donoprasum, anisobrachial chromosomes, mainly sm or st, as well as SAT-chromosomes of the "Codonoprasum type" (m A or sm A ), have been observed in the majority of the studied species of this section (Tzanoudakis 1983(Tzanoudakis , 1992. Conservation status -Based on data currently available and considering the IUCN Red List Categories and Criteria (IUCN 2012), Allium symiacum could possibly be assigned to the category Critically Endangered: CR D. Specifically, the area of occupancy is estimated to be less than 10 km 2 , the species is so far known only from a single location and the number of mature individuals is estimated at fewer than 50. Nevertheless, further field research during the flowering season could provide more information, such as the size and dynamics of the population, as well as the existence of new localities, which could change the category and the conservation status of the species.
Allium symiacum is also well distinguished from A. dirphianum, A. orestis, A. phitosianum, A. rausii and A. tardans by the size and colour of the flowers: in A. symiacum the tepals are shorter than 5 mm and greenish white with a dark green midvein (Fig. 3B), whereas in the former species the tepals are longer than 5 mm and mostly in shades of pink, purple or brown.
Considering the white colour in its flowers, Allium symiacum could also be compared with the autumnflowering Aegean species A. aegilicum, A. apolloniensis, A. archeotrichon and A. platakisii, which, however, are well differentiated from the new species on the basis of their campanulate to semi-cylindric and longer perigon.
Although Allium symiacum is characterized by exserted stamens, it is well differentiated from the Greek species of A. sect. Codonoprasum that have similar flowers and inflorescence, i.e. the species of the A. flavum and A. stamineum groups. Those species have leaves sheathing less than ½ of the stem, perigon yellowish, pinkish or brownish, ovary more or less globose and often stipitate, and they flower in spring or early summer. Allium symiacum shows also some similarity to A. tardiflorum Kollmann & Shmida, another autumn-flowering species described from Israel, mainly in the shape of the inflorescences. The later species, however, is distinct in its much longer flowers and the almost unilateral spathe valves (Kollmann & al. 1990).
On the basis of the above comparisons, Allium symia cum should be considered as a species with a well distinguished taxonomic position among the Greek species of A. sect. Codonoprasum: it is the only species with exserted stamens in the autumn-flowering subgroup of this section and at the same time it is the only autumn-flowering species among those species of this section that have exserted stamens. Moreover, A. symiacum is characterized by leaves sheathing more than ¾ of the stem and greenish white perianth segments less than 5 mm long. Leaves sheathing more than ¾ of the stem and a life cycle in which dormancy is almost absent are characters that coexist in a few other Greek Allium species belonging to three different sections, viz. A. archeotrichon and A. makrianum C. . Characters like these have been considered as biological traits and adaptations in the arid climatic conditions prevailing in the Mediterranean area during the late Tertiary, i.e. more than 5 million years ago, and the species concerned have been considered as relict floristic elements (Greuter 1979;Kollmann & al. 1990;Tzanoudakis & Kypriotakis 1993, 2008Brullo & al. 1997;Tzanoudakis 2000).
Taking into consideration the morphology (leaf sheaths) and the life cycle (autumn -flowering/no dormancy) of Allium symiacum, it could be also considered as a relict element. This aspect is further supported by the symmetrical diploid karyotype and the restricted distribution range. Plant taxa characterized by small size, an unusual flowering period and small population size, however, are rather under-collected and as a consequence their distribution range is often underestimated. This is the case of some Allium species previously considered endemic to Greece, viz. A. aeginiense Brullo & al., A. brussalisii Tzanoud. & Kypr., A. guicciardii Heldr. and A. pilosum Sm., which have been recently reported from Asia Minor (Koçyiğit & al. 2014).