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1 April 1997 Studies of the Genetics and Expression of Prowing (Pw): A Primitive Homeotic Mutant of the German Cockroach, Blattella germanica
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Abstract

The prowing, T (9; 10)/9; 10 Pw, is a homeotic mutant of the German cockroach, Blattella germanica. Adults are characterized by winglike extensions from the pronotum. This trait is associated with a reciprocal translocation of chromosomes 9 and 10. Translocation heterozygotes express the trait and homozygotes are lethal. The expression of Pw is variable in adults. Individuals with excellent expression show that the pronotal extension is undoubtedly a primitive wing, judging from its venation. We crossed adults with different degrees of expression and found that crosses between parents with excellent expression tended to produced F1 with excellent expression and those with poor expression produced F1 with poor the expression. This suggests that more than one factor is involved in the expression of the trait, and that the different expressions cannot be due to different “expressivity”. We also found that the expression Pw was greatly reduced when prowings were crossed with the Nara wild strain, although this did not happen when they were crossed with individuals with wild-type expression from the Pw strain. This suggests that at least one more factor participates in the expression of Pw. We observed meiosis clearly even in the adult stage. We examined many male adults and confirmed that the reciprocal translocation cause Pw expression without an exception. We also observed the frequencies and stages of embryonic death of F1 from both Pw and Pw × wild type parents. The results agreed well with the ratio between alternate and adjacent disjunction at metaphase I. Pw individuals tended to be delay in nymphal development compared with the wild type.

INTRODUCTION

The Pw was first described by Ross (1964), and has been maintained in the Genetic Stock Center for the German Cockroach in the Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA., USA. Genetic studies showed that Pw is inherited as an autosomal, semi-dominant lethal trait (Ross and Cochran, 1965). Subsequently, it was discovered that Pw is associated with a reciprocal translocation between chromosomes 9 and 10, T(9; 10)/9; 10 Pw (Cochran and Ross, 1969). Because pronotal winglets also characterize a deficiency of chromosome 9, it is thought that the trait is associated with the breakage of chromosome 9, (Ross and Cochran, 1971). Pw is characterized by winglike-expansion of the pronotum in the adult stage and a trancheal pattern like that of the meso- and metathoracic wing buds in nymphs. The prowing resembles the paranotal lobes of ancestral insects. Fossil evidence indicates that extinct pterygotes had wing on every thoracic and abdominal segment (Kukalova-Peck, 1978; Carroll et al., 1995). This mutant of the German cockroach is very interesting, since it is a rare case of extant insects bearing a pair of prothoracic wings. It is known that the expressivity of Pw is variable; some individuals have large protrusions that look like wings but others bear only tiny protuberances. However, this expressivity has not been studied quantitativively. Studies of the expressivity may reveal the factors that are involved in the expression of the trait. The purpose of the present study was to examine the combinations of crosses of individual cockroaches with different expressions of Pw and to investigate the meiocytes of the translocation heterozygotes. These date will serve as a foundation for future studies using this variable mutant.

MATERIALS AND METHODS

Maintenance of stock

Translocation heterozygotes T(9;10)/9; 10 Pw are characterized by pronotal winglets. The homozygote is lethal. Thus, both crosses (Pw/+ × Pw/+ and Pw/+ × +/+) can be used for maintenance of the stock. The former cross results in i0289-0003-14-2-339-ex1.gif in the ratio of offspring, but embryonic trapping caused by neighboring dead embryos due to lethal Pw/Pw and adjacent disjunction increases the frequency of embryonic death. The latter cross results in a high percentage of viable offsprings, but the ratio of +/+ increases. Therefore, we used both crosses to maintain the stock. Newly molted adult males with +/+ expression were eliminated twice a week, because males can copulate about 4 days after adult ecdysis. This means that both crosses (Pw/+ male × Pw/+ female) and (Pw/+ male × +/+ female) take place in the stock container. The simple energy-saving method may be the best way to maintain the Pw stain.

Crosses

Newly molted adults were used for crosses in most cases. All females used were newly molted adults of the males, newly molted adults were preferably used as parents. However, a few males with degrees of Pw1 and Pw2 (see below) in Pw expression were used from the stock container, because these degrees were relatively rare and it is sometimes difficult to achieve good timing for mating only newly molted males were to be used. The Pw expression of the parents was categorized into 4 grades: poor (Pw1), intermediate (Pw2), good (Pw3) and excellent (Pw4). Individuals with completely wild type expression from the Pw strain were also used (Pw+). Nara wild individuals (Nara) were also mated with every expression (Pw+ − Pw4) of the Pw strain. For the judgment of F1 expression, degrees were classified into 7 grades: Pw 0, 1, 1, 2, 3, 4 and 4+; in addition to the grades used in the parents, the two grades of marginally poor (1) and exceptional (4+) were added. In the calculation of the average, the degrees of Pw expression 1, 1, 2, 3, 4, and 4+ were given the values 0.5, 1, 2, 3, 4, and 4.5, respectively. To identify the stage of embryonic death, the developmental table of the German cockroach was used (Tanaka, 1976).

Chromosome observation

The testes of the fourth to the last instars and adults were observed. The testes were dissected and then immersed in water for 5 min. to make them swell. The left and right testes were placed separately in droplets of 15% acetic acid on a glass slide, and excess fat body was removed from the testes forceps. The testes were stained with acetic orcein less than 5 min, covered with a cover glass, and pushed gently with the head of a forceps; the excess orcein was thus soaked up. Each testis was then wrapped with tissue paper, put upside down and, pushed forcefully by the examiner's thumb from behind the slide to flatten the testis. Fingernail polish was then spread along the edge of the cover glass. The specimen was observed with a phase contrast microscope.

RESULTS

The expressivity of Pw in adults was variable. Expression was often similar between the left and right sides, as shown in Fig. 1, but sometimes differed between the sides. Individuals without the translocation (+/+) in the Pw stock showed wild type expression, as in Fig. 1a. The translocation heterozygotes showed a large variety of Pw expression (Fig. 1b–f). A few Pw individuals in the Pw stock showed still poorer expression, designated as (1), which was often encountered in the heterozygotes outcrossed to the Nara wild strain. Close examination confirmed the finding of Ross (1964) that postero-lateral protrusions of the pronotum are serially homologous with wings and similar to structures in early fossil insects, although sometimes this seemed doubtful in gross examinations, especially when the degree of expression was poor. Figure 2 shows that veins occur in pronotal winglets like those of the wing (also see Ross 1964, Fig. 5c–d).

Fig. 1

Wild type (a) and various expressions of Pw. Degrees of 1 ~4+ (b) ~(f), respectively. 1, poor; 2, intermediate; 3, good; 4, excellent; 4+, exceptional.

i0289-0003-14-2-339-f01.jpg

Fig. 2

Evidence that a pronotal protrusion is actually a small wing, judging from its venation.

i0289-0003-14-2-339-f02.jpg

Table 1 shows the percentages of Pw expression in F1 individuals between parents with various expression of Pw. Crosses between phenotypical wild type siblings of Pw resulted in no F1 with Pw expression. Reciprocal crosses between Nara wild strain and Pw+ also resulted in no Pw expression in the F1. The percentages of Pw individuals from crosses between the Nara wild and Pw strain with Pw expression fluctuated in the range from 41.0% to 53.3% by the degrees of Pw expression. The average of these was 46.6%, nearly equal to the expected 50%. The percentages of Pw from crosses within the Pw strain between wild type siblings and Pw (Pw 1~4) ranged from 46.5% to 57.6%. The average was 52.3%, also nearly equal to the expected 50%. The percentages of Pw from crosses between parents with Pw expression fluctuated from 59.4% to 83.3% by the degrees of expression. The average of 16 combinations was 72.1% Pw, close to the expected value, 69% (see Discussion).

Table 1

The percentages of Pw individuals in F1 from crosses with various degrees of Pw expression, including outcrosses to Nara wild strain

i0289-0003-14-2-339-t01.gif

In order to study the Pw expressivity between P1 and F1, crosses of various degrees of expression were carried out. Individuals showing the typical degrees +~4 (Fig. 1) were selected as parents. The Nara wild strain was also used as a control of the outcross. In the observation of F1, besides the typical degrees 1~4, very poor expression (Pw1) and exceptional (Pw4+) were encountered. Therefore, in the calculation of the average expression, Pw1 and Pw4+ were given the values 0.5 and 4.5, and Pw1 to Pw4 were assigned the values 1 to 4, respectively. Table 2 summarizes the results of these crosses. In the experiment with parents both with Pw expression, Pw4 × Pw4 resulted in the highest average value (3.53), but less than the parental value of 4. Likewise, Pw4 × Pw3 resulted in the value 3.26, the reciprocal average of 101 offspring (61 from Pw3 male × Pw4 female and 40 from Pw3 female × Pw4 male). The value from the Pw3 × Pw3 cross (2.85) was nearly equal to that of the reciprocal averages of Pw4 × Pw2 (3.01). The next highest values appeared in the reciprocal averages of Pw3 × Pw2 (2.85) and Pw4 × Pw1 (2.55). These values were almost equal to the average of the parents. The value of Pw2 × Pw2 was 2.28, and the reciprocal average of Pw3 × Pw1 was 2.38; both values were slightly higher than the values of the parental average. The next lowest value was found in the Pw2 × Pw1; the reciprocal average was 1.78, also somewhat higher than that of the parents. The lowest average (1.50) was from the Pw1 × Pw1 cross; this value was higher than that of the parents. In the crosses between Pw and wild type siblings (Pw+), the reciprocal averages of Pw+ × Pw1, Pw+ × Pw2, Pw+ × Pw3, and Pw+ × Pw4 were 1.88, 2.42, 2.29, and 3.01, respectively. These values did not suggest any inhibitory factors of Pw expression from Pw+ parents, especially in the crosses of Pw+ × Pw1, and Pw+ × Pw2. In contrast, when Pw1, Pw2, Pw3, and Pw4 were outcrossed to the Nara wild strain, the reciprocal averages much lower, i.e., 0.92, 1.18, 1.24, and 1.08, respectively, suggesting that the Nara wild strain has inhibitory factor(s) on Pw expression. The F1 of Nara wild × Pw+, and Pw+ × Pw+ all showed wild type expression, as expected.

Table 2

Average degrees of Pw expression in F1 from crosses with various degrees of Pw expression, including outcrosses to Nara wild strain

i0289-0003-14-2-339-t02.gif

Table 3 shows the percentages of hatching and deaths at various embryonic stages. Both crosses of Nara wild × Pw+ and Pw+ × Pw+ resulted in high percentages of hatching. The former showed the normal percentage of the Nara wild standard. In the latter, a few embryos (3.42%) died before stage 12; the reason for this unknown. There were no significant differences between Nara wild × Pw (1~4) and Pw+ × Pw (1~4) in the percentages of hatching and embryonic death, in spite of great differences in Pw expression (Table 2). Both crosses showed about 68% hatching, very close to the theoretical value, 67% (see Table 4). The embryonic death rate before stage 17 was about 30%, also close to the theoretical value (33%). In the crosses between parents with Pw expression (1 ~4), 11% of the embryos died by embryonic trapping (Keil and Ross, 1977) due to the death in neighboring compartments of the ootheca. High percentages of embryonic death, 48.21% before or at 12 and 10.33% from st. 13 to st. 17, caused siblings to fail to hatch due to embryonic trapping within the ootheca. The percentage of hatching including those of embryonic trapping reached about 41%, close to the theoretical value, 36% (see Discussion). The total death rate by stage 17 was about 59%, also close to the theoretical value (64%).

Table 3

The percentages of embryos hatched or died at various stages

i0289-0003-14-2-339-t03.gif

Table 4

Zygotic combination from every disjunction type of gamete in the crosses between translocation heterozygotes, T(9; 10)/9: 10 Pw

i0289-0003-14-2-339-t04.gif

The diploid number of chromosomes of Blattella germanica females is 2n = 24, XO type (Suomalainen, 1946). We found that meiosis is easily observable, even in the adult stage. We examined many testes of adult males with or without Pw expression, and found that all translocation heterozygotes show Pw expression and all wild type zygotes have wild type pronota. Figures 3 and 4 show some comparisons between translocated and wild type meiocytes. There was a clear difference at the diplotene stage. Eleven paired chromosomes and an X chromosome were always found in the wild type (Fig. 3a), whereas configurations typical of reciprocal translocations were always found in the Pw males (Fig. 3b). At diakinesis in the wild type, 12 groups of chromosomes were found including X, some as rings, others as rods, according to the number of chiasmata which were already terminalized (Fig. 3c). In contrast, in the Pw at diakinesis, 11 groups of chromosomes were found; one was a large ring consisting of 4 member chromosomes (Fig. 3d). The wild type at metaphase I is shown in Fig. 4a. The Pw meiocytes showed either the adjacent type of disjunction (Fig. 4b) or the alternate type (Fig. 4c), as described by Cochran (1977).

Fig. 3

Meiosis I of Blattella germanica (n=12), (a) and (b), diplotene-early diakinesis; (c) and (d), diakinesis; (a) and (c), wild type; (b) and (d), prowing. A diamond shape (b) and a large ring (d) show reciprocal translocation.

i0289-0003-14-2-339-f03.jpg

Fig. 4

Metaphase I of Blattella germanica. (a) wild type; (b) prowing, adjacent disjunction (rectangle-shaped); (c) prowing, alternate disjunction (Z-shaped in the center of the photo).

i0289-0003-14-2-339-f04.jpg

Fig. 5

Comparison of oothecae. (a) wild type (living); embryos of advanced stage can be seen through the surface of the ootheca in all compartments. (b) Pw × wild type (living); dead embryos (white appearance) are often found (theoretically 33% dead). (c) Pw × Pw (specimen in 80% ethanol); live embryos are dark, dead embryos are light-colored (theoretically 64% dead).

i0289-0003-14-2-339-f05.jpg

Table 4 shows all possible combinations of chromosomes from the matings between translocation heterozygotes. The theoretical average percentage of such undeveloped embryos is 64% (both parents are Pw/+) or 33% (one of parents is +/+) if the alternate:adjacent ratio is 2:1, the same ratio as in the testis and ovary. Dead embryos were easily distinguished from normal ones. Figure 5 shows some examples of oothecae including such embryos together with normal ootheca. Figure 5a is a normal wild type ootheca. An advanced-stage embryo can be seen in each compartment. When one of the parents was Pw, about 1/3 of the embryos died during embryonic development (Fig. 5b). About 2/3 of the embryos died when both parents were Pw (Fig. 5c).

We compared the period of nymphal development between individuals with various degrees of Pw expression and wild type expression (Table 5). The total period from hatching to adult ecdysis was recovered in all individuals. Siblings from the same ootheca were divided into groups: reaching adulthood in the first half period among the siblings, and reaching adulthood in the last half period. The date of the individuals from all oothecae in each type of cross are summarized in Table 5. In every type of cross, the wild type individuals tended to grow faster than those with Pw expression. In the cross of Nara wild to Pw, many F1 with extremely poor expression (1) appeared, and some were delayed in nymphal growth.

Table 5

Comparison of nymphal period of F1 between wild-type and Pw expression

i0289-0003-14-2-339-t05.gif

DISCUSSION

It was found that the expression of Pw is more or less inheritable (Table 2). Therefore, differences in the expression of Pw cannot be thought of as differences of the expressivity of genetically identical individuals. The date in Table 2 suggests that Pw expression may be related to three or more factors. Since Pw is associated with the breakage of chromosome 9 (Ross and Keil, 1978), the site of chromosome breakage may include one or more inhibitory factor(s) of pronotal wings, because normal gene(s) in the site act as inhibitor(s) to completely suppress the primitive trait. The expression of the F1 showed a gradient according to that of their parents in the crosses between two parents with Pw expression. This finding suggests that more than one factor is involved in Pw expression. Some factor(s) might not be in the translocation site. Another factor may exist because the average expression tended to be much less in the outcrosses to Nara wild strain than in the crosses to the +/+ of the Pw strain.

It has been believed that the meiosis of cockroaches is rarely encountered in adult testes and should thus be observed during the nymphal stage. We found here that many meiocytes can be frequently observed in the adult testes. The occurrence of early-stage meiocytes in adult males is possibly a reserve for later matings, since the males copulate several times during their life span. We confirmed the findings on Pw nymphs (Cochran and Ross, 1969) that individuals with Pw expression have translocated chromosomes and those with wild type expression have wild type chromosomes. The penetrance is thus 100%.

The ratios between alternate and adjacent disjunction differ among mutants of translocation heterozygotes. It is known that the frequency of alternate disjunction in the prowing mutant is 63~64% in male nymphs (Cochran and Ross, 1974; Cochran, 1977). Table 4 shows all zygotic combinations with the ratio in supposition that the alternate:adjacent ratio is about 2:1, close to the previous finding in the testes (Cochran and Ross, 1974). The observation of meiocytes in ovaries is almost impossible due to the large amount of yolk. Therefore, the same ratio was assumed in the ovary to make this table. The ratio of +/+:Pw/+:Pw/Pw (lethal):deficiencies (lethal) is 16:36:16:76 under the supposition of alt.:adj.=2:1 in both male and female meiocytes. The percentages are theoretically 11%, 25%, 11%, and 53%, respectively. The average Pw expression of F1 from the Pw/+ × Pw/+ crosses shown in Table 1 was 72.1%, which agrees well with the theoretical estimates of 69% and 67.1% from an earlier study (Ross and Cochran, 1965). Pw/Pw may correspond to late embryonic death, and chromosomal deficiency may cause early embryonic death. This supposition also explains the results shown in Table 3 that the incidence of late embryonic death was 10.33% and that of early embryonic death was 48.21%. The first column of Table 4 shows the results that would be expected from matings between Pw/+ and +/+. The ratio of offspring of +/+, Pw/+, and deficiencies (lethal) are 33% each. Thus, the expected ratio of viable offspring between +/+ and Pw/+ is 1:1. This explains the results of Tables 1 and 5 that the frequency of Pw was 46.6% in Nara wild × Pw/+ and 52.3% in Pw/+ × Pw/+. Both were close to 50% and agree with the data of Ross and Cochran (1965). The percentages of embryonic death as of stage 17, about 30% in both Nara wild × Pw/+ and +/+ × Pw/+ (Table 3), are also explained by the date in the first column of Table 4.

From the viewpoint that Pw expresses a primitive developmental pathway, it is of interest that the loci of other primitive traits such as notched sternite (st) (Ross, 1966), miniature-wing (min) (Ross and Keil, 1978), stumpy (sty) (Ross, 1975; Tanaka and Ross, 1989), and maxillary-palp-elongate (mpe) (Ross and Tanaka, 1988; Tanaka and Ross, 1990) are on the same chromosome. Concerning the origin of insect wings, the pronotal wings of the German cockroach seems to favor the paranotal hypothesis (Hinton, 1963; Rasnitsyn, 1981), although other evidence seems to support the pleural hypothesis (Wigglesworth, 1963; Kukalova-Peck, 1983).

Acknowledgments

We thank Mary H. Ross for sending us the prowing mutant and for reading the manuscript. We also thank Mina Andou for her help in preparing the manuscript.

REFERENCES

1.

S. B. Carroll, S. D. Weatherbee, and J. A. Langeland . 1995. Homeotic genes and the regulation and evolution of insect wing number. Nature 375:58–61. Google Scholar

2.

D. G. Cochran and M. H. Ross . 1969. Chromosome identification in the German cockroach, wild type and mutant stocks. J Hered 60:87–92. Google Scholar

3.

D. G. Cochran and M. H. Ross . 1974. Cytology and genetics of T (9, 11) in the German cockroach, and its relationship to other chromosome 9 traits. Can J Genet Cytol 16:639–649. Google Scholar

4.

D. G. Cochran 1977. Patterns of disjunction frequencies in heterozygous reciprocal translocations from the German cockroach. Chromosoma 62:191–198. Google Scholar

5.

H. E. Hinton 1963. The origin of flight in insect. Proc R Entomol Soc London Ser C 28:24–25. Google Scholar

6.

C. B. Keil and M. H. Ross . 1977. An analysis of embryonic trapping in the German cockroach. Entomol Exp Appl 22:220–226. Google Scholar

7.

J. Kukalova-Peck 1978. Origin and evolution of insect wings and their relation to metamorphosis as documented by the fossil record. J Morphol 156:53–125. Google Scholar

8.

J. Kukalova-Peck 1983. Origin of the insect wing and wing articulation from the arthropodan leg. Can J Zool 61:1618–1669. Google Scholar

9.

A. P. Rasnitsyn 1981. A modified paranotal theory of insect wing origin. J Morphol 168:331–338. Google Scholar

10.

M. H. Ross 1964. Pronotal wings in Blattella germanica (L.) and their possible evolutionary significance. Amer Mid Natur 71:161–180. Google Scholar

11.

M. H. Ross and D. G. Cochran . 1965. A preliminary report on genetic variability in the German cockroach, Blattella germanica. Ann Entomol Soc Am 58:368–375. Google Scholar

12.

M. H. Ross 1966. Notched sternite: a mutant of Blattella germanica, with possible implications for the homology and ventral abdominal structures. Ann Entomol Soc Amer 59:473–484. Google Scholar

13.

M. H. Ross and D. G. Cochran . 1971. Cytology and genetics of a pronotalwing trait in the German cockroach. Can J Genet Cytol 13:522–535. Google Scholar

14.

M. H. Ross 1975. Genetic variability in the German cockroach. X. Genetics of pale purple, pearl, and stumpy. J Hered 66:155–159. Google Scholar

15.

M. H. Ross and C. B. Keil . 1978. Genetic variability in the German cockroach. XI. Does chromosome 9 carry remnants of a primitive gene system. J Hered 69:337–340. Google Scholar

16.

M. H. Ross and A. Tanaka . 1988. Genetic variability in the German cockroach. XII: A third mutant that suggests chromosome 9 carries a highly conserved group of closely linked genes. J Hered 79:439–443. Google Scholar

17.

E. Suomalainen 1946. Die chromosomenverhaltnisse in der spermatogenese einiger Blattarien. Ann Acad Sci Fenn 4:1–60. Google Scholar

18.

A. Tanaka 1976. Stages in the embryonic development of the German cockroach, Blattella germanica Linné (Blattaria, Blattellidae). Kontyû 44:512–525. Google Scholar

19.

A. Tanaka and M. H. Ross . 1989. Tibia to femur ratios of unaltered and regenerated legs of the stumpy mutant of the German cockroach. Zool Sci 6:927–933. Google Scholar

20.

A. Tanaka and M. H. Ross . 1990. Instability of the number of segments of unoperated and regenerated maxillary palpi in the maxillary-palp-elongate (mpe) German cockroach mutant. Zool Sci 7:671–679. Google Scholar

21.

V. B. Wigglesworth 1963. The origin of flight in insects. Proc R Entomol Soc London Ser C 28:23–32. Google Scholar
Akira Tanaka and Tamiyo Ito "Studies of the Genetics and Expression of Prowing (Pw): A Primitive Homeotic Mutant of the German Cockroach, Blattella germanica," Zoological Science 14(2), 339-346, (1 April 1997). https://doi.org/10.2108/zsj.14.339
Received: 3 February 1997; Accepted: 1 February 1997; Published: 1 April 1997
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