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1 August 2006 A Rapid Increase in cAMP in Response to 20-Hydroxyecdysone in the Anterior Silk Glands of the Silkworm, Bombyx mori
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Abstract
In the anterior silk glands (ASGs) of the silkworm, Bombyx mori, intracellular cAMP increases transiently to a very high level shortly after the hemolymph ecdysteroid peak in the prepupal period. In cultured ASGs obtained on the day of gut-purge, cAMP levels were increased by 20-hydroxy-ecdysone (20E), and this increase was enhanced by an inhibitor of phosphodiesterase, but was not affected by α-amanitin, indicating the 20E action may not be mediated via gene expression. The increase in cAMP occurred within 30 seconds of exposure to a physiological concentration of 20E (1 μM), and also by ponasterone A. Our findings indicate a nongenomic action of ecdysteroids in insects, which may be an additional mechanism by which this steroid hormone induces acute responses in tissues and cells.
Mohamed Elmogy, Jun Terashima, Masatoshi Iga, Masafumi Iwami and Sho Sakurai "A Rapid Increase in cAMP in Response to 20-Hydroxyecdysone in the Anterior Silk Glands of the Silkworm, Bombyx mori," Zoological Science 23(8), (1 August 2006). https://doi.org/10.2108/zsj.23.715
Received: 10 March 2006; Accepted: 1 April 2006; Published: 1 August 2006
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