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1 June 2010 Functional Characteristics of Amphioxus Troponin in Regulation of Muscle Contraction
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Abstract

Troponin regulates contraction of vertebrate striated muscle in a Ca2 -dependent manner. More specifically, it acts as an inhibitor of actin-myosin interaction in the absence of Ca2 during contraction. In vertebrates, this regulatory mechanism is unlike that in some less highly derived taxa. Troponin in the smooth muscle of the protochordate ascidian species Halocynthia roretzi regulates actinmyosin contraction as an activator in the presence of Ca2 , not as an inhibitor in the absence of Ca2 as is the case in vertebrates. In this study, contractile regulation of striated muscle from another protochordate, the amphioxus Branchiostoma belcheri, was analyzed using recombinant troponin components TnT, TnI, and TnC that were produced in an Escherichia coli expression system to further elucidate their roles in Ca2 -dependent regulation of the actin-myosin interaction. Combination of these troponin components in an actin-myosin ATPase activity assay showed that troponin in amphioxus striated muscle functions in a similar manner to troponin in vertebrate striated muscle, and differently from ascidian smooth muscle troponin. Thus, troponin function appears to have evolved differently in different protochordate muscles.

© 2010 Zoological Society of Japan
Jeanette G. Dennisson, Yukiko Tando, Naruki Sato, Michio Ogasawara, Kaoru Kubokawa, and Takashi Obinata "Functional Characteristics of Amphioxus Troponin in Regulation of Muscle Contraction," Zoological Science 27(6), 461-469, (1 June 2010). https://doi.org/10.2108/zsj.27.461
Received: 29 November 2009; Accepted: 1 January 2010; Published: 1 June 2010
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