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1 September 2011 Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene
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Abstract

The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney.

© 2011 Zoological Society of Japan
Jin-Han Bae, Kung Ahn, Gyu-Hwi Nam, Chong-Eon Lee, Kyung-Do Park, Hak-Kyo Lee, Byung-Wook Cho, and Heui-Soo Kim "Molecular Characterization of Alternative Transcripts of the Horse BMAL1 Gene," Zoological Science 28(9), 671-675, (1 September 2011). https://doi.org/10.2108/zsj.28.671
Received: 11 November 2010; Accepted: 1 February 2011; Published: 1 September 2011
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