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1 December 2014 Molecular Cloning of Two Structure Variants of Crustacean Hyperglycemic Hormone (CHH) from the Swimming Crab (Portunus trituberculatus), and Their Gene Expression During Molting and Ovarian Development
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Abstract

Two full-length cDNA (Pt-CHH1 and Pt-CHH2) sequences encoding crustacean hyperglycemic hormone (CHH) were cloned from tissues of the swimming crab (Portunus trituberculatus) using RACE. Pt-CHH1 was cloned from eyestalk, whereas Pt-CHH2 was cloned from thoracic ganglia. Sequence and structure analyses of Pt-CHH1 and Pt-CHH2 suggest that they may be generated from alternative splicing. Tissue distribution showed that transcript of Pt-CHH1 was only detected in eyestalk, while transcript of Pt-CHH2 was observed in several extra-eyestalk tissues. The transcript levels of Pt-CHH1 and Pt-CHH2 during molting and ovarian development were determined using qPCR. In molting process, level of Pt-CHH1 in eyestalk increased from stage A (postmolt), and to significant higher at stage C (intermolt), then decreased during premolt (D0–D4). In ovarian development, level of Pt-CHH1 in eyestalk decreased from previtellogenic stage (II), and to significant lower at mature stage (IV). The expression patterns of Pt-CHH2 in thoracic ganglia and Y-organ were distinct from that of Pt-CHH1 in eyestalk. The combined results suggest that Pt-CHH1 may be involved in inhibition of molting and ovarian development, whereas Pt-CHH2 may have other physiological functions.

© 2014 Zoological Society of Japan
Xi Xie, Dongfa Zhu, Jifen Yang, Xier Qiu, Xiaoyu Cui, and Jie Tang "Molecular Cloning of Two Structure Variants of Crustacean Hyperglycemic Hormone (CHH) from the Swimming Crab (Portunus trituberculatus), and Their Gene Expression During Molting and Ovarian Development," Zoological Science 31(12), 802-809, (1 December 2014). https://doi.org/10.2108/zs140053
Received: 12 March 2014; Accepted: 1 August 2014; Published: 1 December 2014
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