We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30–35°N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. We propose the karyotype of this coral (2n = 30) based on C-banding and FISH analyses. FISH mapping of the rRNA gene was carried out with a probe generated by PCR amplification using rRNA gene primers. Furthermore, the telomeres and centromeres of all chromosomes were visualized using FISH. By comparative genomic hybridization using DNA from sperm and unfertilized eggs of this coral, we offer evidence suggesting the existence of sex chromosomes in this species. Collectively, these data advance our understanding of coral genetics.
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1 February 2014
Molecular Cytogenetic Analysis of the Scleractinian Coral Acropora solitaryensis Veron & Wallace 1984
Takahiro Taguchi,
Takuma Mezaki,
Fumihito Iwase,
Satoko Sekida,
Satoshi Kubota,
Hironobu Fukami,
Kazuo Okuda,
Teruyuki Shinbo,
Syun-ichirou Oshima,
Yoshiaki Iiguni,
Joseph R Testa,
Akira Tominaga
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Zoological Science
Vol. 31 • No. 2
February 2014
Vol. 31 • No. 2
February 2014
CGH
Chromosome
Coral
Fish
rDNA
telomere
