De Novo Assembly of the Transcriptome of Turritopsis, a Jellyfish that Repeatedly Rejuvenates

Yoshinori Hasegawa; Takashi Watanabe; Masaki Takazawa; Osamu Ohara; Shin Kubota

doi:10.2108/zs150186

Translator Disclaimer

1 August 2016 De Novo Assembly of the Transcriptome of Turritopsis, a Jellyfish that Repeatedly Rejuvenates

Yoshinori Hasegawa, Takashi Watanabe, Masaki Takazawa, Osamu Ohara, Shin Kubota

Author Affiliations +

Zoological Science, 33(4):366-371 (2016). https://doi.org/10.2108/zs150186

Abstract

In most animals, aging is an irreversible process; however the species Turritopsis sp. has been observed to undergo a rejuvenation process as many as 14 times. In the present study, we used multiplexed RNA libraries to obtain the transcriptome from four developmental stages (St) of Turritopsis sp., including (I) immature medusa, (II) dumpling, (III) dumpling with a short stolon, and (IV) polyp, which had recently rejuvenated. A total of 4.02 billion paired-end reads were assembled de novo, yielding 90,327 contigs. Our analyses revealed that significant blast hits were recovered for 74% of the assembled contigs, and 19% were successfully annotated with gene ontology (GO) terms. A BLAST search demonstrated that 32% of the contigs were most similar to Hydra vulgarissequences. Raw reads from each sample were mapped against the contigs to find St-specific genes. This represents the first comprehensive set of de novo transcriptome data for this species, which may provide clues toward a better understanding of cyclical rejuvenation in multicellular animals.

Citation Download Citation

Yoshinori Hasegawa, Takashi Watanabe, Masaki Takazawa, Osamu Ohara and Shin Kubota "De Novo Assembly of the Transcriptome of Turritopsis, a Jellyfish that Repeatedly Rejuvenates," Zoological Science 33(4), (1 August 2016). https://doi.org/10.2108/zs150186

Received: 17 November 2015; Accepted: 1 April 2016; Published: 1 August 2016

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

Price: $10.00 ADD TO CART

Includes PDF & HTML, when available

RIGHTS & PERMISSIONS

KEYWORDS/PHRASES

PUBLICATION TITLE:

PUBLICATION YEARS