Natural plant extracts, especially essential oils, are used largely against plant pests. The aim of this study was to investigate the bio-insecticidal effect of chemical extracts of three plants, Mentha pulegium L. (Lamiales: Lamiaceae), Lavandula stoechas L. (Lamiales: Lamiaceae) and Ruta chalepensis L. (Sapindales: Rutaceae) collected from Tunisia, on the lesser grain borer Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae), which is a primary pest of stored wheat. To reach this goal, we firstly did bio-assays to test the toxicity of these plant extracts on R. dominica adults using different concentrations of plant extracts on wheat seeds. Secondly, we analysed the chemical compounds of these plant extracts using GC-MS and GC-FID. Our results demonstrated that R. dominica mortality was significantly higher with increasing plant essential oils concentrations (CL₉₅ values 45.68, 113.12 and 170.52 µl/l_(air)) for M. pulegium, R. chalepensis and L. stoechas, respectively. The main chemical compounds identified of M. pulegium oils were: pulegone (76.9 %), isomenthone (12.0 %) and limonene (1.73 %), whereas those from L. stoechas were: camphor (23.8 %), 1,8-cineole (17.8 %) and camphene (7.69 %). Ruta chalepensis oil revealed high contents of undecanone (51.2 %), 2-nonanone (39.16 %) and 2-decanone (2.32 %). Our results also showed that M. pulegium essential oil has a significant effect against R. dominica with LC₅₀ value of 13.51 µl/l_(air) compared to R. chalepensis and L. stoechas corresponding to 39.11 and 42.9 µl/l_(air), respectively.