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1 April 2009 Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides
Xiaoqiong Qi, Yongxia Yang, Yingjuan Su, Ting Wang
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Abstract

A new full-length genomic DNA, encoding a member of the cyanovirin-N (CV-N) homologous protein family, has been cloned from the fern species Ceratopteris thalictroides by chromosome walking. It is 1993 bp long, contains a 723 bp open reading frame (ORF) that encodes a deduced protein (named CtCVNH) with 150 amino acid residues. CtCVNH has a predicted isoelectric point (PI) of 4.47 and a calculated molecular mass 15.9556 kDa. It possesses the conserved anti-HIV (human immunodeficiency virus) CV-N domain, which is the same as the cyanovirin-N homology (CVNH) members that were isolated from filamentous ascomycetes and C. richardii. Modeling of the tertiary structure indicated that CtCVNH is an elongated, largely β-sheet protein that displays internal two-fold pseudosymmetry. Comparative structure analysis of the predicted CtCVNH with native CV-N revealed that the major evolutionary changes occurring during the evolution of plant CVNHs were: 1) a length increase at N- and C-terminal regions; and 2) a loop to helix transition at the helical-turn regions. Phylogenetic analysis showed that CtCVNH was grouped together with the two CVNHs from C. richardii.

Xiaoqiong Qi, Yongxia Yang, Yingjuan Su, and Ting Wang "Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides," American Fern Journal 99(2), 78-92, (1 April 2009). https://doi.org/10.1640/0002-8444-99.2.78
Published: 1 April 2009
JOURNAL ARTICLE
15 PAGES


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KEYWORDS
bioinformatic analysis
Ceratopteris thalictroides
chromosome walking
CVNH
inverse PCR
single oligonucleotide nested PCR
thermal asymmetric interlaced PCR
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