Spores are commonly used to start in vitro culture of ferns. Numerous methods for spore surface sterilization and sowing have been developed, but spore loss and contamination are still problematic. To overcome these problems, an efficient method for sterilizing and sowing spores was established. Through this method, contamination and loss of spores is minimized, and can be sown in adjustable, even densities.
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in vitro culture
spore sowing
surface sterilization