This study reports a new hybrid combination Ophrys × circlarium Pellegrino, hybr. nov., which derives from two highly divergent species, O. lutea (O. fusca-lutea complex) and O. tarentina (O. sphegodes complex). These two species grow sympatrically in the north of Calabria region (southern Italy), in a stand where two potential hybrid individuals were found during a floristic investigation. Two of the 16 morphometric characters analyzed were intermediate relative to those of the potential parental species. PCR-RFLP analysis of nuclear ribosomal DNA Internal Transcribed Spacer sequences (rDNA ITS) confirmed that the two specimens are hybrids of the two cooccurring Ophrys species.
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Vol. 45 • No. 1